All documents related the the HBGS course Tags in protein expression, detection and purification. Most documents are available in both PDF and OpenOffice format. Feel free to repurpose the documents. They are licensed under the Creative Commons Attribution-Noncommercial-Share Alike 1.0 License.

Lecture notes

Tag use in large scale protein purification
PDF format: tags_in_large_scale_production.pdf
OpenOffice format: tags_in_large_scale_production.odp

(Incomplete) list of protein tags used for purification
Gnumeric format: Protein_tag_list_incomplete.gnumeric

Protocols

Protocols & schedule for the practical part of the course
PDF format: Purification_of_secreted_protein_from_cell_sups.pdf
OpenOffice format: Purification_of_secreted_protein_from_cell_sups.odt

Quite good handbook from Qiagen about purification of histagged proteins with Qiagen Ni2+NTA
PDF format: The_QIAexpressionist.pdf

Results

Chromatograms of the purifications
PDF format: High5-VEGF-D_chromatogram_group1.pdf
OpenOffice format: High5-VEGF-D_chromatogram_group1.odg
PDF format: CHO-VEGF-C_chromatogram_group2.pdf
OpenOffice format: CHO-VEGF-C_chromatogram_group2.odg

Chromatogram of the gel filtration (2nd purification step) of VEGF-D
PDF format: High5-VEGF-D_GF_chromatogram.pdf
OpenOffice format: High5-VEGF-D_GF_chromatogram.odg

Silver-stained PAGE gel of VEGF-D gel filtration
PDF format: High5-VEGF-D_gel_filtration.pdf 

Silver-stained PAGE gel of VEGF-D and VEGF-C IMAC
PDF format: High5-VEGF-D+CHO-VEGF-C_IMAC.pdf

Zipped Source files (tif for the images and Inkscape svg for the vector graphics) of the PAGE gel images
PDF format: sources.zip